XF^e Extracellular Flux Analyzers

The World's Most Advanced Cell Metabolism Analyzers

XF^e Extracellular Flux Analyzers are the Gold Standard platform for metabolic assays today and in the future, simultaneously measuring the two major energy producing pathways of the cell - mitochondrial respiration and glycolysis - in a microplate, in real-time. The XF^e96, XF^e24 and XF24-3 Analyzers, stress test kits, and reagents can determine in vitro oxygen consumption, glycolysis, CO₂, mitochondrial dysfunction, and fatty acid oxidation.

This fast and sensitive measurement of cellular bioenergetics is label-free, enabling time-resolved analysis and the reuse of the cells. XF assays provide increased throughput in a drug discovery format that is superior to its single parameter predecessors. By incorporating hardware, software, and apps that are made for each other, low-cost, disposable fluorescent optical sensors, multiplexed fiber optics and highly integrated electronic components - XF^e instruments offer scientists the ability to:

• Perform time-resolved measurements of a single population of cells over a period of minutes, hours or even days.
• XF^e24 and XF^e96 Analyzers simultaneously measure oxygen consumption rate (OCR), and extracellular acidification rate (ECAR).
• XF24-3 Analyzer simultaneously measures carbon dioxide evolution rates (CDER), and oxygen consumption rates (OCR), in isolated mitochondria or whole cells.
• Reuse cell samples after completing an XF measurement.

A Complete System

The XF^e Extracellular Flux Analyzer, XF stress test kits, reagents, consumables, and software apps provide the easiest and most comprehensive assessment of cell metabolism available in a microplate today. XF^e Analyzers are highly-sophisticated, yet easy-to-use instruments comprised of hardware, software, and apps including:

Bench-Top Analyzer

The XF^e Analyzer fits easily on a bench-top and requires only a standard lab power connection. An internal measuring chamber is maintained at 37°C at all times, and plates can be loaded manually, or a robotic plate handler and fluidics system can be used to develop automated assay protocols.

User-Friendly Controller Interface

A high-resolution, color LCD touch-screen provides a rich user interface for increased productivity and shorter assay running times. Integrated with the XF^e Analyzer, the networkable controller comes loaded with Microsoft Windows® Operating System.

XF^e Wave

XF^e Wave is software that supports the way that you work, taking advantage of today's technological advances to simplify your assay workflow and analysis, with multi-touch screen capability. An easy 4-step process guides you through your assay, and provides a succinct design summary report on completion. Experiments can be designed on any desktop computer, and then imported into the XF^e Analyzer in your lab. XF^e data can be viewed in raw form, and then analyzed by exporting to Excel™, or by using the XF^e software review and charting tools. XF^e Wave comes with an unlimited-use license for your organization.

XF^e Analyzers are used in conjunction with the XF Prep Station - required with the XF^e96 Analyzer, and optional with the XF^e24 Analyzer.

XF Stress Test Kits & Reagents

XF stress test kits and regents provide a standardized means to perform XF assays quickly and easily. Pre-calibrated, quality assured reagents, and standard assay protocols, ensure reliable and reproducible data.

Convenient XF^e FluxPaks

Each XF^e Analyzer comes with two XF^e FluxPaks which contain the correct ratio of XF^e Assay Kits, XF Cell Culture Plates and XF Calibrant necessary to perform up to eighteen 24-well or 96-well assays.

• How XF Technology Works
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• Instruments Overview
  • XF Analyzers
  • XF Prep Station
• Consumables
  • XF Kits & Reagents
  • FluxPaks
  • XF Microplates
  • XF Media
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• XF Ordering
  • Request a Quote
  • Rental Option
  • Grant Expeditor Package
  • Online Consumables Ordering
  • Distributors