XF Instruments Overview
All XF instruments integrate label free technology, eliminating label artifacts in cellular responses and drug interactions. XF analyzers also incorporate a disposable assay cartridge design, facilitating better tissue culture techniques, requiring no special clean-up, and saving time.
| Instrument | Features | Benefits |
|---|---|---|
XFe96![]() |
96 well format, O2 and H+ rates, 4 drug injection chambers. Learn More |
The XFe96 Analyzer, 96-well format simultaneously measures mitochondrial respiration and glycolysis in cells, in minutes. The XFe96 provides high-throughput capability at the lowest per sample cost. The XFe96 also requires fewer cells and lower reagent volumes than the 24-well format, while enabling a larger number of experimental groups per assay. Ideal for labs seeking to maximize metabolic data acquisition, while conserving precious samples and reagents. |
XFe24![]() |
24 well format, O2 and H+ rates, 4 drug injection chambers. Learn More |
The XFe24 Analyzer, 24-well format also simultaneously measures mitochondrial respiration and glycolysis. The multiple injection ports allow for cell manipulations, and large plate bottom diameter enables compatibility among many biological sample types including islets and isolated mitochondria, among others. The XFe24 is ideal for labs doing initial assay development, with smaller cell metabolism experimental workloads. |
XF24-3![]() |
24 well format, O2, H+, and CO2, 4 drug injection chambers. Learn More |
For labs wanting the ability to measure of a third analyte for supplemental metabolic pathway information, the XF24-3 Analyzer enables the measurement of the TCA cycle, in addition to mitochondrial respiration and glycolysis. The XF24-3 Analyzer utilizes XF24-3 FluxPaks to measure carbon dioxide evolution rates (CDER), and oxygen consumption rates (OCR) simultaneously, in isolated mitochondria or whole cells. |
XF Prep Station![]() |
96 or 24 well format, non CO2 incubator, media warmer and washer Learn More |
Efficiently removes growth medium replacing with run medium. Improves data CVs and H+ measurement sensitivity while minimizing sample prep time. Required for XFe96. Recommended for XFe24. |
Next Seahorse Webinar
Metabolic Adaptations in Fumarate Hydratase Deficient Cells

Christian Frezza, PhD,
Hutchison/MRC Research Centre, Cambridge, UK
May 29, 2013
Advanced XF Training
Understanding When and How to Use Intact Cells and Isolated Mitochondria in Investigating Bioenergetics
Learn More
XF Stress Test Kits
XF Glycolysis Stress Test Kit
Now it's easy to measure glycolytic function in cells.
Learn MoreXF Cell Mito Stress Test Kit
Everything you need to measure mitochondrial function in cells.
Learn More



