XF Cell Mito Stress Test Kit Buy Now

XF Cell Mito Stress Test Kit

Everything you need to measure mitochondrial function in cells

Part # 101706‐100

Measurements of mitochondrial function are essential to understanding cancer, aging, and metabolic, cardiovascular, and neurodegenerative diseases. The XF Cell Mito Stress Test Kit makes it easy to measure the four key parameters of mitochondrial function in a microplate: basal respiration, ATP turnover, proton leak, and maximal respiration, revealing critical information not evident in basal metabolism measurements alone.

Now you can confidently quantify cellular bioenergetics, identify mitochondrial function, and measure cells' response to stress.

  • Complete, easy-to-use kit for 6 microplates
  • Software wizard guides assay optimization, design, and interpretation
  • Designed for the XF Analyzers

Using the pre-calibrated, pre-tested reagents, the XF Cell Mito Stress Test Kit reveals the complete mitochondrial stress profile, while new algorithms automatically calculate the most important metrics, simplifying your work.

Measure basal respiration, ATP turnover, proton leak, and spare respiratory capacity.

XF Cell Mito Stress Test Profile

Mito Stress Test

The fundamental parameters of mitochondrial function: basal respiration, ATP turnover, proton leak, and maximal respiration, or spare respiratory capacity.

Each XF Cell Mito Stress Test Kit eliminates the questions and concerns of where to source the reagents, what concentrations are optimal, and best storage conditions. Each convenient kit contains enough reagents to run six XF24 or XF96 microplates, allowing you to run a full mitochondrial profile in each well. With the reagents now ready at your fingertips, the XF Cell Mito Stress Test Kit is your ultimate tool for assay optimization.

Specifications

  • Pre-calibrated, pre-tested reagents:
    • Oligomycin (ATP Coupler)
    • FCCP (ETC Accelerator)
    • Antimycin A (Mito Inhibitor A)
    • Rotenone (Mito Inhibitor B)
  • DMSO (Solvent)

XF Technology Impacts Cellular Bioenergetics Research

Cancer

Cancer

Cancer. 2011 Jun 30;
Individualizing antimetabolic treatment strategies for head and neck squamous cell carcinoma based on TP53 mutational status
Sandulache VC, Skinner HD, Ow TJ, Zhang A, Xia X, Luchak JM, Wong LJ, Pickering CR, Zhou G, Myers JN.

Aging

Nature

Nature. 2009 May 21; 459(7245):387-92.
Bmi1 regulates mitochondrial function and the DNA damage response pathway
Liu J, Cao L, Chen J, Song S, Lee IH, Quijano C, Liu H, Keyvanfar K, Chen H, Cao LY, Ahn BH, Kumar NG, Rovira, Ii, Xu XL, Van Lohuizen M, Motoyama N, Deng CX, Finkel

Neurodegeneration

Neurochem

J Neurochem. 2011 Jul; 118(1):79-92.
Mitochondrial aconitase knockdown attenuates paraquat-induced dopaminergic cell death via decreased cellular metabolism and release of iron and H2 O2
Cantu D, Fulton RE, Drechsel DA, Patel M.

Obesity, Diabetes,
& Metabolic Disorders

Ophthalmol

Invest Ophthalmol Vis Sci. 2011 Oct 6 [Epub ahead of print]
High Glucose Induces Mitochondrial Morphology and Metabolic Changes in Retinal Pericytes
Trudeau K, Molina AJ, Roy S.

Cardiovascular

Molecular Cell

BBA Molecular Cell Research, 2011 Aug 4
Susceptibility to simvastatin-induced toxicity is partly determined by mitochondrial respiration and phosphorylation state of Akt
Mullen PJ, Zahno A, Lindinger P, Maseneni S, Felser A, Krähenbühl S, Brecht K.

Stem Cell Biology

Nature

Nature. 2010 Dec 2;468(7324):659-63.
The Lkb1 metabolic sensor maintains haematopoietic stem cell survival.
Gurumurthy S, Xie SZ, Alagesan B, Kim J, Yusuf RZ, Saez B, Tzatsos A, Ozsolak F, Milos P, Ferrari F, Park PJ, Shirihai OS, Scadden DT, Bardeesy N.