XF Cell Mito Stress Test Kit
Everything you need to measure mitochondrial function in cells
Part # 101706‐100
Measurements of mitochondrial function are essential to understanding cancer, aging, and metabolic, cardiovascular, and neurodegenerative diseases. The XF Cell Mito Stress Test Kit makes it easy to measure the four key parameters of mitochondrial function in a microplate: basal respiration, ATP turnover, proton leak, and maximal respiration, revealing critical information not evident in basal metabolism measurements alone.
Now you can confidently quantify cellular bioenergetics, identify mitochondrial function, and measure cells' response to stress.
- Complete, easy-to-use kit for 6 microplates
- Software wizard guides assay optimization, design, and interpretation
- Designed for the XF Analyzers
Using the pre-calibrated, pre-tested reagents, the XF Cell Mito Stress Test Kit reveals the complete mitochondrial stress profile, while new algorithms automatically calculate the most important metrics, simplifying your work.
Measure basal respiration, ATP turnover, proton leak, and spare respiratory capacity.
Each XF Cell Mito Stress Test Kit eliminates the questions and concerns of where to source the reagents, what concentrations are optimal, and best storage conditions. Each convenient kit contains enough reagents to run six XF24 or XF96 microplates, allowing you to run a full mitochondrial profile in each well. With the reagents now ready at your fingertips, the XF Cell Mito Stress Test Kit is your ultimate tool for assay optimization.
- Pre-calibrated, pre-tested reagents:
- Oligomycin (ATP Coupler)
- FCCP (ETC Accelerator)
- Antimycin A (Mito Inhibitor A)
- Rotenone (Mito Inhibitor B)
- DMSO (Solvent)
XF Technology Impacts Cellular Bioenergetics Research
Cancer. 2011 Jun 30;
Nature. 2009 May 21; 459(7245):387-92.
J Neurochem. 2011 Jul; 118(1):79-92.
BBA Molecular Cell Research, 2011 Aug 4
Stem Cell Biology
Nature. 2010 Dec 2;468(7324):659-63.
- How XF Technology Works
- Instruments Overview
- XF Ordering
Next Seahorse Webinar
Christian Frezza, PhD,
Hutchison/MRC Research Centre, Cambridge, UK
May 29, 2013
Advanced XF Training
Understanding When and How to Use Intact Cells and Isolated Mitochondria in Investigating Bioenergetics
XFe Extracellular Flux Analyzers
The world's most advanced cell metabolism analyzer.Learn More
Learn More with these on-Demand Webinars
- Profiling mitochondrial function has never been so easy: A tutorial on the new XF Cell Mito Stress Test Kit
-David Ferrick, PhD, CSO, Seahorse Bioscience
- Mitochondrial profiling in cells under physiological conditions and in response to stress
-Victor Darley-Usmar, PhD, University of Alabama at Birmingham
- Spare respiratory capacity and the bioenergetics of the nerve terminal
-David Nicholls PhD, FRSE, Buck Institute for Age Research & Lund University
- The rate and coupling efficiency of oxidative phosphorylation in mitochondria and cells
-Martin Brand, PhD, Buck Institute & MRC Dunn Nutrition Unit