Screening Anti-Ischemic Drugs with a Gas-Flow Modified XF Analyzer
Paul S. Brookes, PhD
University of Rochester Medical Center
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Originally presented May 16, 2012
Ischemia and/or hypoxia of cells and tissues is an important pathologic process in many human diseases including heart attack, stroke, and cancer. By making simple modifications to the XF Analyzer and XF Assay cartridges, we developed a system that enables gas-flow inside the instrument, thus affording the ability to expose cells to different pO2 conditions.
Using this system, we recently screened a library of small molecules, to identify compounds that protect cells from ischemic injury.
In this webinar we will discuss:
- Overview of the practicalities of adapting the XF Analyzer for gas flow.
- The relationship between effects of drugs on ischemic injury, and their effects on metabolism.
- Considerations when performing high throughput screening, such as pooling strategies, heat maps, edge effect, and drug library bias.
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Hutchison/MRC Research Centre, Cambridge, UK
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