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XF Glycolysis Stress Test Assesses Glycolysis and Glycolytic Capacity in Cells

Guest Presenter:

Min Wu, PhD
Seahorse Bioscience

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Originally presented August 30, 2011

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Webinar Abstract:

Glycolysis is involved in a wide array of normal physiological processes [stem cells pluripotency, acquired immune response, muscle function, brain function], as well as pathophysiological processes [tumorigenesis].

Glycolysis consists of a series of biochemical reactions which convert glucose to lactate producing ATP and results in a net production of protons. As glycolysis occurs, cells in culture release protons and acidify the culture medium. The Seahorse XF Extracellular Flux Analyzer measures the change in proton concentration of the medium surrounding cells in real-time -- the extracellular acidification rate or ECAR -- as a measure of the glycolytic activity of the cell.

We have developed a quick and easy test, the Glycolytic Stress Test, that assesses glycolysis and glycolytic capacity of a variety of cell types with the XF Analyzer.

In this webinar we will discuss:

How Extracellular Acidification measures cell glycolysis
How to use the Extracellular Acidification Rate (ECAR) in parallel with oxygen consumption (OCR) to monitor cellular bioenergetics in real-time
How the Glycolysis Stress Test makes assessing glycolytic function of cells easy