XF Glycolysis Stress Test Assesses Glycolysis and Glycolytic Capacity in Cells
Min Wu, PhD
If you are having trouble logging in, please email our Webmaster for help.
Originally presented August 30, 2011
Glycolysis is involved in a wide array of normal physiological processes [stem cells pluripotency, acquired immune response, muscle function, brain function], as well as pathophysiological processes [tumorigenesis].
Glycolysis consists of a series of biochemical reactions which convert glucose to lactate producing ATP and results in a net production of protons. As glycolysis occurs, cells in culture release protons and acidify the culture medium. The Seahorse XF Extracellular Flux Analyzer measures the change in proton concentration of the medium surrounding cells in real-time -- the extracellular acidification rate or ECAR -- as a measure of the glycolytic activity of the cell.
We have developed a quick and easy test, the Glycolytic Stress Test, that assesses glycolysis and glycolytic capacity of a variety of cell types with the XF Analyzer.
In this webinar we will discuss:
- How Extracellular Acidification measures cell glycolysis
- How to use the Extracellular Acidification Rate (ECAR) in parallel with oxygen consumption (OCR) to monitor cellular bioenergetics in real-time
- How the Glycolysis Stress Test makes assessing glycolytic function of cells easy
- Glycolytic Function
- Stem Cells
- Video Library
- App Notes & Tech Briefs
- Product Documentation & Literature
- Protocols & Best Practices
- Technical Posters
- Cell Line Reference
- Core Facilities
- XF Instrument Map
- Frequently Asked Questions
- Image Library
- Useful Links
Next Seahorse Webinar
Christian Frezza, PhD,
Hutchison/MRC Research Centre, Cambridge, UK
May 29, 2013
Advanced XF Training
Understanding When and How to Use Intact Cells and Isolated Mitochondria in Investigating Bioenergetics
XFe Extracellular Flux Analyzers
The world's most advanced cell metabolism analyzer.Learn More