Mitochondrial Profiling in Cells Under Physiological Conditions and In Response to Stress
Victor Darley-Usmar, PhD
University of Alabama at Birmingham
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Originally presented August 4, 2010
It is well known that mitochondria provide intermediary metabolites and serve the bioenergetic needs of a wide variety of cells. The extracellular flux technology is particularly well suited for assessing how these functions impact on oxidative phosphorylation and glycolysis. A particularly useful and straight-forward protocol has been to add in series an inhibitor of the ATP synthase (oligomycin), an uncoupler (FCCP) and a respiratory chain inhibitor (antimycin or rotenone) to obtain mitochondrial profile of cells under basal and stressed conditions.
In this webinar we will discuss:
- How this mitochondrial profiling can be used to determine the response of cells to oxidative stress.
- How cellular differentiation can modify the mitochondrial profile.
- How the basal measurement of oxygen consumption can be combined with the measurement of glycolysis to gain a metabolic image of the cells response to stressors.
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Next Seahorse Webinar
Christian Frezza, PhD,
Hutchison/MRC Research Centre, Cambridge, UK
May 29, 2013
Advanced XF Training
Understanding When and How to Use Intact Cells and Isolated Mitochondria in Investigating Bioenergetics
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