Custom Microplates
Contact Us
Webinar Log-in

Cellular Bioenergetics Webinar Log-in

Email Address

Password

Remember Me

Forgot Password

Don't have a username or password? Register now for our free webinar series.

Products
Products

All XF instruments integrate label free technology, eliminating label artifacts in cellular responses and drug interactions.
- XF Ordering
- Custom Microplates
Applications
Applications

Seahorse XF instruments measure energy utilization in living cells, simultaneously quantifying mitochondrial respiration and glycolysis in real time.
Resources
Resources

Seahorse XF technology has been adopted by leading academic institutions, pharmaceutical, and biotech organizations around the world.
Support
Support

We offer comprehensive customer support ranging from instrument installation and service to specific biological application support of XF technology.
Ordering
Ordering

Initiate the purchase of your XF^e Analyzer with a quote or rental option, as well as place your orders for XF Stress Test Kits, and other consumables.
- Online Consumables Ordering
- Distributors
Company
Company

Providing industry-leading analytical instruments, biomanufacturing systems and consumable labware products for biological research and drug discovery.

Mitochondrial Profiling in Cells Under Physiological Conditions and In Response to Stress

Guest Presenter:

Victor Darley-Usmar, PhD
University of Alabama at Birmingham

If you are having trouble logging in, please email our Webmaster for help.

Originally presented August 4, 2010

Log In to View webinar on-Demand. New users can Register now.

Webinar Abstract:

It is well known that mitochondria provide intermediary metabolites and serve the bioenergetic needs of a wide variety of cells. The extracellular flux technology is particularly well suited for assessing how these functions impact on oxidative phosphorylation and glycolysis. A particularly useful and straight-forward protocol has been to add in series an inhibitor of the ATP synthase (oligomycin), an uncoupler (FCCP) and a respiratory chain inhibitor (antimycin or rotenone) to obtain mitochondrial profile of cells under basal and stressed conditions.

In this webinar we will discuss:

How this mitochondrial profiling can be used to determine the response of cells to oxidative stress.
How cellular differentiation can modify the mitochondrial profile.
How the basal measurement of oxygen consumption can be combined with the measurement of glycolysis to gain a metabolic image of the cells response to stressors.