Achieving Higher Throughput and Greater Content When Measuring Mitochondrial Function
David Ferrick, PhD
CSO, Seahorse Bioscience
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Originally presented January 22, 2009
The introduction of Extracellular Flux (XF) technology in 2006 enabled non-invasive, real time profiling of cellular bioenergetics and mitochondrial function in lving cells. This opened a new window to study cellular metabolism. And the ability to assess primary, engineered and genetically altered cells under various substrates conditions or drug exposures became easier.
Now, over 200 scientists routinely rely on Seahorse XF analyzers. With the advent of the 96-well based system, screening for compounds or pathways that alter mitochondria function and overall bioenergetic behavior can now be routinely accomplished. As well, basic studies can benefit by the additional throughput so that many more conditions such as multiple substrates and genetic alterations can be run in a single experiment.
In this webinar we will discuss:
- How easy it is to generate 96 wells of cellular bioenergetic data.
- How precise and accurate XF data is.
- How to take advantage of the much greater throughput in designing bioenergetic experiments.
- Energy Expenditure: Flux: Mitochondrial Respiration; Glycolysis
- Mitochondrial Function: BOFA
- Toxicology & Hepatobiology
- Video Library
- App Notes & Tech Briefs
- Product Documentation & Literature
- Protocols & Best Practices
- Technical Posters
- Cell Line Reference
- Core Facilities
- XF Instrument Map
- Frequently Asked Questions
- Image Library
- Useful Links
Next Seahorse Webinar
Christian Frezza, PhD,
Hutchison/MRC Research Centre, Cambridge, UK
May 29, 2013
Advanced XF Training
Understanding When and How to Use Intact Cells and Isolated Mitochondria in Investigating Bioenergetics
XFe Extracellular Flux Analyzers
The world's most advanced cell metabolism analyzer.Learn More