Application Notes & Technical Briefs

Application Notes and Technical Briefs written by cellular bioenergetic scientists for cellular bioenergetic researchers. These articles offer experienced analysis, assay ideas, and reference diagrams to assist you with your research. Can be viewed online or download as a printable PDF.

App Notes

    (Summary of 3 to 4 publications utilizing XF technology in an application area)

• Functional validation of Genomic and Proteomic data
• Assessing mitochondrial dysfunction in primary cardiomyocytes
• Bioenergetic Analysis of Primary Neuronal Deficiencies
• Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes
• Defining metabolic phenotypes in cancer cells
• Direct Comparison of Radiometric versus XF24 Flux Assays to Measure Substrate Utilization
• Measuring bioenergetic profiles of human adipocytes
• Measuring fatty acid oxidation in muscle cells
• Measuring glycolysis and oxidative metabolism in cancer cells
• Metabolic Pathway Determination Using the XF24 Analyzer
• Normalizing XF metabolic data to cellular or mitochondrial parameters
• Screening for Mitochondrial Biogenesis and Toxicity
• Understanding the Relationship Between Bioenergetic Rates and ATP Turnover

Tech Briefs

    (Guidelines to modify standard XF assays for different samples)

• Analyzing microgram quantities of isolated mitochondria in the XF24 Analyzer
• Measuring mitochondrial defects in human skin fibroblasts
• Measuring mitochondrial respiration in intact skeletal muscle fibers

• Overview
•  
• Webinars
  • Upcoming Webinars
  • On-Demand Webinars
  •  
  • Log-in
  • Register
• Video Library
•  
• App Notes & Tech Briefs
• Product Documentation & Literature
• Protocols & Best Practices
• Publications
• Technical Posters
•  
• Cell Line Reference
• Core Facilities
• XF Instrument Map
•  
• Frequently Asked Questions
• Image Library
• Useful Links

Next Seahorse Webinar

Metabolic Adaptations in Fumarate Hydratase Deficient Cells

Christian Frezza, PhD,
Hutchison/MRC Research Centre, Cambridge, UK
May 29, 2013

Learn More See More Events

Advanced XF Training

Understanding When and How to Use Intact Cells and Isolated Mitochondria in Investigating Bioenergetics

Learn More

XF^e Extracellular Flux Analyzers

The world's most advanced cell metabolism analyzer.

Learn More

XF Stress Test Kits

XF Glycolysis Stress Test Kit

Now it's easy to measure glycolytic function in cells.

Learn More

XF Cell Mito Stress Test Kit

Everything you need to measure mitochondrial function in cells.

Learn More

previous next