Best Practices & Protocols
Protocols
Protocols developed by Seahorse cellular bioenergetic scientists for cellular bioenergetic researchers are available to cover a variety of applications.
These protocols offer step-by-step instructions to prepare and run standard XF assays, and can be viewed online or downloaded as a printable PDF.
- 3T3-L1 Pre-Adipocyte Differentiation
- Immobilization of Non-Adherent Cells with Cell-Tak™ for Assay on the XF Analyzer
- Simultaneous measurement of the TCA cycle and respiration in isolated mitochondria with the XF24-3 Analyzer and CO2 sensor cartridge
- Simultaneous measurement of the TCA cycle and respiration in cells with the XF24-3 Analyzer and CO2 sensor cartridge
- Islet Assay using the XF24 Islet Capture Microplate
- Preparation of Bovine Serum Albumin (BSA)-Conjugated Palmitate
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Next Seahorse Webinar
Metabolic Adaptations in Fumarate Hydratase Deficient Cells
Christian Frezza, PhD,
Hutchison/MRC Research Centre, Cambridge, UK
May 29, 2013
Advanced XF Training
Understanding When and How to Use Intact Cells and Isolated Mitochondria in Investigating Bioenergetics
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XF Stress Test Kits
XF Glycolysis Stress Test Kit
Now it's easy to measure glycolytic function in cells.
Learn MoreXF Cell Mito Stress Test Kit
Everything you need to measure mitochondrial function in cells.
Learn More