Applications in Stem Cell Biology
App Notes & Tech Briefs
A real time assay that quantifies the ATP and biosynthetic demands of immune cell proliferation, differentiation and effector function.
Normal suspension cells, such as hematopoietic cells and lymphocytes are responsible for supplying oxygen to the body and protecting the host. Cellular metabolism is markedly dynamic in immune responses during proliferation and immune effector functions...Learn More
A reduction in ATP demand and mitochondrial activity with neural differentiation of human embryonic stem cells
J Cell Sci; 2011 Feb 1. 124 (Pt 3):348-358.
PLoS One; 2011 Jun 17. 6 (6):e20914.
Stem Cells; 2011 Jul 1. 29 (7):1064-1074.
Human iPSCs Harbor Homoplasmic and Heteroplasmic Mitochondrial DNA Mutations While Maintaining hESC-Like Metabolic Reprogramming
Stem Cells; 2011 Sep 1. 29 (9):1338-1348.
Proc Natl Acad Sci U S A; 2011 Sep 20. 108 (38):16062-16067.
Cell Metab; 2011 Oct 5. 14 (4):537-544.
Mitochondrial development of the in vitro hepatic organogenesis model with simultaneous cardiac mesoderm differentiation from murine induced pluripotent stem cells
J Biosci Bioeng; 2011 Nov 1. 112 (5):495-500.
EMBO J; 2011 Nov 15. 30 (24):4860-4873.
A critical role of mitochondrial phosphatase ptpmt1 in embryogenesis reveals a mitochondrial metabolic stress-induced differentiation checkpoint in embryonic stem cells
Mol Cell Biol; 2011 Dec 1. 31 (24):4902-4916.
Human Pompe disease induced pluripotent stem cells for pathogenesis modeling, drug testing and disease marker identification
Hum Mol Genet; 2011 Dec 15. 20 (24):4851-64.
Am J Physiol Cell Physiol; 2012 Jan 1. 302 (2):C463-472.
The metabolome of induced pluripotent stem cells reveals metabolic changes occurring in somatic cell reprogramming
Cell Res; 2012 Jan 1. 22 (1):168-77.
Nat Cell Biol; 2012 Jan 15. 14 (2):209-19.
HIFalpha induced switch from bivalent to exclusively glycolytic metabolism during ESC--EpiSC transition
EMBO Journal; 2012 Mar 23. 31 (9):2103-16.
Culture of human mesenchymal stem cells at low oxygen tension improves growth and genetic stability by activating glycolysis
Cell Death Differ; 2012 May 1. 19 (5):743-55.
Cell Stem Cell; 2012 May 4. 10 (5):515-519.
Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells
Nat Protoc; 2012 Jun 1. 7 (6):1068-1085.
Pharmacological Rescue of Mitochondrial Deficits in iPSC-Derived Neural Cells from Patients with Familial Parkinson's Disease
Sci Transl Med; 2012 Jul 4. 4 (141):141ra190.
Cell Stem Cell; 2012 Aug 3. 11 (2):253-63.
Mouse hematopoietic cell-targeted STAT3 deletion: stem/progenitor cell defects, mitochondrial dysfunction, ROS overproduction, and a rapid aging-like phenotype
Blood; 2012 Sep 27. 120 (13):2589-99.
Regulation of glycolysis by pdk functions as a metabolic checkpoint for cell cycle quiescence in hematopoietic stem cells
Cell Stem Cell; 2013 Jan 3. 12 (1):49-61.
Metabolic regulation by the mitochondrial phosphatase PTPMT1 is required for hematopoietic stem cell differentiation
Cell Stem Cell; 2013 Jan 3. 12 (1):62-74.
BCL-2 Inhibition Targets Oxidative Phosphorylation and Selectively Eradicates Quiescent Human Leukemia Stem Cells
Cell Stem Cell. 2013 Jan 15; [Epub ahead of print].
Stem Cells. 2013 Jan 17; [Epub ahead of print].
Cellular Bioenergetics is an Important Determinant of the Molecular Imaging Signal Derived from Luciferase and the Sodium-Iodide Symporter
Circ Res; 2013 Feb 1. 112 (3):441-50.
Stem Cells. 2013 Feb 8; [Epub ahead of print].
The TBC1D15 Oncoprotein Controls Stem Cell Self-Renewal through Destabilization of the Numb-p53 Complex
PLoS One; 2013 Feb 27. 8 (2):e57312.
Resveratrol Impedes the Stemness, Epithelial-Mesenchymal Transition, and Metabolic Reprogramming of Cancer Stem Cells in Nasopharyngeal Carcinoma through p53 Activation
Evidence-Based Complementary and Alternative Medicine. 2013 Mar 16; [Epub ahead of print].
Tissue Eng Part A. 2013 Apr 30; [Epub ahead of print].
20th Annual International Society for Magnetic Resonance in Medicine (ISMRM) Meeting
May 5-11, 2012
Targeting Mitochondria 2010
November 18-19, 2010
Stem cell biology is of prime importance in regenerative medicine, and the understanding of aging and age-related disorders, and related therapies. Recent studies have highlighted the significance of genetic and environmental modulation of mitochondria and cellular metabolism, whereby these factors are integral in the maintenance and differentiation of stem cells.
The XF Analyzer generates data that can provide new understanding in relation to stem cell biology and mitochondrial dysfunction, by measuring cellular metabolism in real-time. By measuring the oxygen consumption rate (OCR) – an indication of mitochondrial respiration; as well as the extracellular acidification rate (ECAR) - a measure of glycolysis, you will be provided with the most physiologically relevant in vitro measurement for your bioenergetic studies.
- Cell Physiology
- Mitochondrial Diseases
- Model Organisms
- Obesity, Diabetes, & Metabolic Disorders
- Stem Cell Biology
- Toxicology & Hepatobiology
- Translational Medicine
Next Seahorse Webinar
Christian Frezza, PhD,
Hutchison/MRC Research Centre, Cambridge, UK
May 29, 2013
Advanced XF Training
Understanding When and How to Use Intact Cells and Isolated Mitochondria in Investigating Bioenergetics
XFe Extracellular Flux Analyzers
The world's most advanced cell metabolism analyzer.Learn More