Applications in Stem Cell Biology

App Notes & Tech Briefs

• Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes

  A real time assay that quantifies the ATP and biosynthetic demands of immune cell proliferation, differentiation and effector function.

  Normal suspension cells, such as hematopoietic cells and lymphocytes are responsible for supplying oxygen to the body and protecting the host. Cellular metabolism is markedly dynamic in immune responses during proliferation and immune effector functions...

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Publications

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• A reduction in ATP demand and mitochondrial activity with neural differentiation of human embryonic stem cells

  Birket M J, Orr A L, Gerencser A A, Madden D T, Vitelli C, Swistowski A, Brand M D and Zeng X

  J Cell Sci; 2011 Feb 1. 124 (Pt 3):348-358.

• Energy metabolism in human pluripotent stem cells and their differentiated counterparts

  Varum S, Rodrigues A S, Moura M B, Momcilovic O, Easley C A t, Ramalho-Santos J, Van Houten B and Schatten G

  PLoS One; 2011 Jun 17. 6 (6):e20914.

• Mitochondria determine the differentiation potential of cardiac mesoangioblasts

  San Martin N, Cervera A M, Cordova C, Covarello D, McCreath K J and Galvez B G

  Stem Cells; 2011 Jul 1. 29 (7):1064-1074.

• Human iPSCs Harbor Homoplasmic and Heteroplasmic Mitochondrial DNA Mutations While Maintaining hESC-Like Metabolic Reprogramming

  Prigione A, Lichtner B, Kuhl H, Struys E A, Wamelink M, Lehrach H, Ralser M, Timmermann B and Adjaye J

  Stem Cells; 2011 Sep 1. 29 (9):1338-1348.

• Metabolic state of glioma stem cells and nontumorigenic cells

  Vlashi E, Lagadec C, Vergnes L, Matsutani T, Masui K, Poulou M, Popescu R, Della Donna L, Evers P, Dekmezian C, Reue K, Christofk H, Mischel P S and Pajonk F

  Proc Natl Acad Sci U S A; 2011 Sep 20. 108 (38):16062-16067.

• Mitochondrial Complex III ROS Regulate Adipocyte Differentiation

  Tormos K V, Anso E, Hamanaka R B, Eisenbart J, Joseph J, Kalyanaraman B and Chandel N S

  Cell Metab; 2011 Oct 5. 14 (4):537-544.

• Mitochondrial development of the in vitro hepatic organogenesis model with simultaneous cardiac mesoderm differentiation from murine induced pluripotent stem cells

  Tamai M, Yamashita A and Tagawa Y I

  J Biosci Bioeng; 2011 Nov 1. 112 (5):495-500.

• UCP2 regulates energy metabolism and differentiation potential of human pluripotent stem cells

  Zhang J, Khvorostov I, Hong J S, Oktay Y, Vergnes L, Nuebel E, Wahjudi P N, Setoguchi K, Wang G, Do A, Jung H J, McCaffery J M, Kurland I J, Reue K, Lee W N, Koehler C M and Teitell M A

  EMBO J; 2011 Nov 15. 30 (24):4860-4873.

• A critical role of mitochondrial phosphatase ptpmt1 in embryogenesis reveals a mitochondrial metabolic stress-induced differentiation checkpoint in embryonic stem cells

  Shen J, Liu X, Yu W M, Liu J, Groot Nibbelink M, Guo C, Finkel T and Qu C K

  Mol Cell Biol; 2011 Dec 1. 31 (24):4902-4916.

• Human Pompe disease induced pluripotent stem cells for pathogenesis modeling, drug testing and disease marker identification

  Huang H P, Chen P H, Hwu W L, Chuang C Y, Chien Y H, Stone L, Chien C L, Li L T, Chiang S C, Chen H F, Ho H N, Chen C H and Kuo H C

  Hum Mol Genet; 2011 Dec 15. 20 (24):4851-64.

• Triiodothyronine induces UCP-1 expression and mitochondrial biogenesis in human adipocytes

  Lee J Y, Takahashi N, Yasubuchi M, Kim Y I, Hashizaki H, Kim M J, Sakamoto T, Goto T and Kawada T

  Am J Physiol Cell Physiol; 2012 Jan 1. 302 (2):C463-472.

• The metabolome of induced pluripotent stem cells reveals metabolic changes occurring in somatic cell reprogramming

  Panopoulos A D, Yanes O, Ruiz S, Kida Y S, Diep D, Tautenhahn R, Herrerias A, Batchelder E M, Plongthongkum N, Lutz M, Berggren W T, Zhang K, Evans R M, Siuzdak G and Belmonte J C

  Cell Res; 2012 Jan 1. 22 (1):168-77.

• Programming human pluripotent stem cells into white and brown adipocytes

  Ahfeldt T, Schinzel R T, Lee Y K, Hendrickson D, Kaplan A, Lum D H, Camahort R, Xia F, Shay J, Rhee E P, Clish C B, Deo R C, Shen T, Lau F H, Cowley A, Mowrer G, Al-Siddiqi H, Nahrendorf M, Musunuru K, Gerszten R E, Rinn J L and Cowan C A

  Nat Cell Biol; 2012 Jan 15. 14 (2):209-19.

• HIFalpha induced switch from bivalent to exclusively glycolytic metabolism during ESC--EpiSC transition

  Zhou W, Choi M, Margineantu D, Margaretha L, Cavanaugh C, Blau C A, Horwitz M S, Hockenbery D M, Ware C and Ruohola-Baker H

  EMBO Journal; 2012 Mar 23. 31 (9):2103-16.

• Culture of human mesenchymal stem cells at low oxygen tension improves growth and genetic stability by activating glycolysis

  Estrada J C, Albo C, Benguria A, Dopazo A, Lopez-Romero P, Carrera-Quintanar L, Roche E, Clemente E P, Enriquez J A, Bernad A and Samper E

  Cell Death Differ; 2012 May 1. 19 (5):743-55.

• Short-term calorie restriction enhances skeletal muscle stem cell function

  Cerletti M, Jang Y C, Finley L W, Haigis M C and Wagers A J

  Cell Stem Cell; 2012 May 4. 10 (5):515-519.

• Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells

  Zhang J, Nuebel E, Wisidagama D R, Setoguchi K, Hong J S, Van Horn C M, Imam S S, Vergnes L, Malone C S, Koehler C M and Teitell M A

  Nat Protoc; 2012 Jun 1. 7 (6):1068-1085.

• Pharmacological Rescue of Mitochondrial Deficits in iPSC-Derived Neural Cells from Patients with Familial Parkinson's Disease

  Cooper O, Seo H, Andrabi S, Guardia-Laguarta C, Graziotto J, Sundberg M, McLean J R, Carrillo-Reid L, Xie Z, Osborn T, Hargus G, Deleidi M, Lawson T, Bogetofte H, Perez-Torres E, Clark L, Moskowitz C, Mazzulli J, Chen L, Volpicelli-Daley L, Romero N, Jiang H, Uitti R J, Huang Z, Opala G, Scarffe L A, Dawson V L, Klein C, Feng J, Ross O A, Trojanowski J Q, Lee V M, Marder K, Surmeier D J, Wszolek Z K, Przedborski S, Krainc D, Dawson T M and Isacson O

  Sci Transl Med; 2012 Jul 4. 4 (141):141ra190.

• Genetic Correction of Huntington's Disease Phenotypes in Induced Pluripotent Stem Cells

  An M C, Zhang N, Scott G, Montoro D, Wittkop T, Mooney S, Melov S and Ellerby L M

  Cell Stem Cell; 2012 Aug 3. 11 (2):253-63.

• Mouse hematopoietic cell-targeted STAT3 deletion: stem/progenitor cell defects, mitochondrial dysfunction, ROS overproduction, and a rapid aging-like phenotype

  Mantel C, Messina-Graham S, Moh A, Cooper S, Hangoc G, Fu X Y and Broxmeyer H E

  Blood; 2012 Sep 27. 120 (13):2589-99.

• Regulation of glycolysis by pdk functions as a metabolic checkpoint for cell cycle quiescence in hematopoietic stem cells

  Takubo K, Nagamatsu G, Kobayashi C I, Nakamura-Ishizu A, Kobayashi H, Ikeda E, Goda N, Rahimi Y, Johnson R S, Soga T, Hirao A, Suematsu M and Suda T

  Cell Stem Cell; 2013 Jan 3. 12 (1):49-61.

• Metabolic regulation by the mitochondrial phosphatase PTPMT1 is required for hematopoietic stem cell differentiation

  Yu W M, Liu X, Shen J, Jovanovic O, Pohl E E, Gerson S L, Finkel T, Broxmeyer H E and Qu C K

  Cell Stem Cell; 2013 Jan 3. 12 (1):62-74.

• BCL-2 Inhibition Targets Oxidative Phosphorylation and Selectively Eradicates Quiescent Human Leukemia Stem Cells

  Lagadinou E D, Sach A, Callahan K, Rossi R M, Neering S J, Minhajuddin M, Ashton J M, Pei S, Grose V, O'Dwyer K M, Liesveld J L, Brookes P S, Becker M W and Jordan C T

  Cell Stem Cell. 2013 Jan 15; [Epub ahead of print].

• Protein O-GlcNAcylation is a Novel Cytoprotective Signal in Cardiac Stem Cells

  Zafir A, Readnower R, Long B W, McCracken J, Aird A, Alvarez A, Cummins T D, Li Q, Hill B G, Bhatnagar A, Prabhu S D, Bolli R and Jones S P

  Stem Cells. 2013 Jan 17; [Epub ahead of print].

• Cellular Bioenergetics is an Important Determinant of the Molecular Imaging Signal Derived from Luciferase and the Sodium-Iodide Symporter

  Chang C, Chan A, Lin X, Higuchi T, Terrovitis J, Afzal J M, Rittenbach A, Sun D, Vakrou S, Woldemichael K, O'Rourke B, Wahl R, Pomper M, Tsui B and Abraham M R

  Circ Res; 2013 Feb 1. 112 (3):441-50.

• Induced Pluripotent Stem Cells with a Pathological Mitochondrial DNA Deletion

  Cherry A B, Gagne K E, McLoughlin E M, Baccei A, Gorman B, Hartung O, Miller J D, Zhang J, Zon R L, Ince T A, Neufeld E J, Lerou P H, Fleming M D, Daley G Q and Agarwal S

  Stem Cells. 2013 Feb 8; [Epub ahead of print].

• The TBC1D15 Oncoprotein Controls Stem Cell Self-Renewal through Destabilization of the Numb-p53 Complex

  Feldman D E, Chen C, Punj V and Machida K

  PLoS One; 2013 Feb 27. 8 (2):e57312.

• Resveratrol Impedes the Stemness, Epithelial-Mesenchymal Transition, and Metabolic Reprogramming of Cancer Stem Cells in Nasopharyngeal Carcinoma through p53 Activation

  Shen Y-A, Hung P-H, Chi W-H, Wang C-Y, Hsieh Y-T, Wei Y H and Chen Y-J

  Evidence-Based Complementary and Alternative Medicine. 2013 Mar 16; [Epub ahead of print].

• Enhanced tissue production through redox control in stem cell-laden hydrogels

  Reid B, Afzal J, McCartney A, Abraham R, O'Rourke B and Elisseeff J

  Tissue Eng Part A. 2013 Apr 30; [Epub ahead of print].

Technical Posters

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• Metabolic Changes of Human Embryonic Stem Cells During Cardiomyocyte Differentiation

  Anna V. Naumova, Daciana H. Margineantu, Kareen L. Kreutziger, Nathan Palpant, James Fugate, Charles E. Murry

  20th Annual International Society for Magnetic Resonance in Medicine (ISMRM) Meeting
  May 5-11, 2012
  Melbourne, AU

• Assessing mitochondrial and cellular bioenergetics in human pluripotent stem cells

  Alessandro Prigione and James Adjay

  Targeting Mitochondria 2010
  November 18-19, 2010
  Berlin, DE

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