Applications in Screening
App Notes & Tech Briefs
A sensitive and robust cell-based screening assay.
Mitochondrial damage compromises ATP production and consequentially cellular viability. Given the central role that mitochondria play in regulating cellular function, drugs that undermine mitochondrial function frequently elicit toxic effects...Learn More
Drug Discov Today; 2008 Mar 1. 13 (40669):268-274.
In vitro assessment of mitochondrial dysfunction and cytotoxicity of nefazodone, trazodone, and buspirone
Toxicol Sci; 2008 Jun 1. 103 (2):335-345.
Biguanide-induced mitochondrial dysfunction yields increased lactate production and cytotoxicity of aerobically-poised HepG2 cells and human hepatocytes in vitro
Toxicol Appl Pharmacol; 2008 Dec 1. 233 (2):203-210.
A computational screen for regulators of oxidative phosphorylation implicates SLIRP in mitochondrial RNA homeostasis
PLoS Genet; 2009 Aug 1. 5 (8):e1000590.
Nutrient-sensitized screening for drugs that shift energy metabolism from mitochondrial respiration to glycolysis
Nat Biotechnol; 2010 Mar 1. 28 (3):249-255.
Anal Biochem; 2010 Sep 1. 404 (1):75-81.
9-oxo-10(E),12(E)-octadecadienoic acid derived from tomato is a potent PPAR α agonist to decrease triglyceride accumulation in mouse primary hepatocytes
Mol Nutr Food Res; 2011 Apr 1. 55 (4):585-93.
PLoS One; 2012 Mar 29. 7 (3):e33755.
J Pharmacol Exp Ther; 2012 Jul 1. 342 (1):106-18.
Assessment of drug-induced mitochondrial dysfunction via altered cellular respiration and acidification measured in a 96-well platform
J Bioenerg Biomembr; 2012 Aug 1. 44 (4):421-37.
PLoS One; 2012 Aug 2. 7 (8):e42484.
Anal Chem; 2013 Jan 2. 85 (1):283-91.
High-throughput screening (HTS) assay for identifying potent and selective small-molecule inhibitors of NADPH oxidases
Society for Free Radical Biology and Medicine (SFRBM 2012)
November 15-18, 2012
San Diego, CA
Analysis of glycolytic flux as a rapid screen to identify low lactate producing CHO cell lines with desirable monoclonal antibody yield and glycan profile
ESACT (European Society for Animal Cell Technology)
May 15-18, 2011
Craig C. Beeson, PhD, Medical University of South Carolina
March 23, 2011
Preclinical screening for drug-induced mitochondrial dysfunction can predict toxicity and reduce attrition (or late stage failure) during drug development. Some new drugs may reach the market and their toxicity may not be discovered until many patients have been exposed. The unpredicted toxicity is due to the drug's adverse effects on mitochondrial function.
The XF Analyzer can facilitate preclinical drug evaluation for potential mitochondrial toxicity, addressing the issue early in the drug development process. The XF Analyzer generates data that can provide new understanding in relation to mitochondrial dysfunction, by allowing the measurement of cellular metabolism in real-time, in a microplate. By measuring the oxygen consumption rate (OCR)—a measure of mitochondrial respiration; as well as the extracellular acidification rate (ECAR)—a measure of glycolysis, you will be provided with the most physiologically relevant in vitro measurement for your bioenergetic studies.
- Cell Physiology
- Mitochondrial Diseases
- Model Organisms
- Obesity, Diabetes, & Metabolic Disorders
- Stem Cell Biology
- Toxicology & Hepatobiology
- Translational Medicine
Next Seahorse Webinar
Christian Frezza, PhD,
Hutchison/MRC Research Centre, Cambridge, UK
May 29, 2013
Advanced XF Training
Understanding When and How to Use Intact Cells and Isolated Mitochondria in Investigating Bioenergetics
XFe Extracellular Flux Analyzers
The world's most advanced cell metabolism analyzer.Learn More