Applications in Immunology

App Notes & Tech Briefs

• Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes

  A real time assay that quantifies the ATP and biosynthetic demands of immune cell proliferation, differentiation and effector function.

  Normal suspension cells, such as hematopoietic cells and lymphocytes are responsible for supplying oxygen to the body and protecting the host. Cellular metabolism is markedly dynamic in immune responses during proliferation and immune effector functions...

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Publications

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• HVCN1 modulates BCR signal strength via regulation of BCR-dependent generation of reactive oxygen species

  Capasso M, Bhamrah M K, Henley T, Boyd R S, Langlais C, Cain K, Dinsdale D, Pulford K, Khan M, Musset B, Cherny V V, Morgan D, Gascoyne R D, Vigorito E, DeCoursey T E, MacLennan I C and Dyer M J

  Nat Immunol; 2010 Mar 1. 11 (3):265-272.

• Fast kinase domain-containing protein 3 is a mitochondrial protein essential for cellular respiration

  Simarro M, Gimenez-Cassina A, Kedersha N, Lazaro J B, Adelmant G O, Marto J A, Rhee K, Tisdale S, Danial N, Benarafa C, Orduna A and Anderson P

  Biochem Biophys Res Commun; 2010 Oct 22. 401 (3):440-446.

• The Lkb1 metabolic sensor maintains haematopoietic stem cell survival

  Gurumurthy S, Xie S Z, Alagesan B, Kim J, Yusuf R Z, Saez B, Tzatsos A, Ozsolak F, Milos P, Ferrari F, Park P J, Shirihai O S, Scadden D T and Bardeesy N

  Nature; 2010 Dec 2. 468 (7324):659-663.

• Expression, signaling proficiency, and stimulatory function of the NKG2D lymphocyte receptor in human cancer cells

  Benitez A C, Dai Z, Mann H H, Reeves R S, Margineantu D H, Gooley T A, Groh V and Spies T

  Proc Natl Acad Sci U S A; 2011 Mar 8. 108 (10):4081-4086.

• Mitochondrial reactive oxygen species promote production of proinflammatory cytokines and are elevated in TNFR1-associated periodic syndrome (TRAPS)

  Bulua A C, Simon A, Maddipati R, Pelletier M, Park H, Kim K Y, Sack M N, Kastner D L and Siegel R M

  J Exp Med; 2011 Mar 14. 208 (3):519-533.

• Glutathionylation acts as a control switch for uncoupling proteins UCP2 and UCP3

  Mailloux R J, Seifert E L, Bouillaud F, Aguer C, Collins S and Harper M E

  J Biol Chem; 2011 Jun 17. 286 (24):21865-21875.

• The Transcription Factor Myc Controls Metabolic Reprogramming upon T Lymphocyte Activation

  Wang R, Dillon C P, Shi L Z, Milasta S, Carter R, Finkelstein D, McCormick L L, Fitzgerald P, Chi H, Munger J and Green D R

  Immunity; 2011 Dec 23. 35 (6):871-882.

• Mitochondrial Respiratory Capacity Is a Critical Regulator of CD8(+) T Cell Memory Development

  van der Windt G J, Everts B, Chang C H, Curtis J D, Freitas T C, Amiel E, Pearce E J and Pearce E L

  Immunity; 2012 Jan 27. 36 (1):38-78.

• Oxidized Mitochondrial DNA Activates the NLRP3 Inflammasome during Apoptosis

  Shimada K, Crother T R, Karlin J, Dagvadorj J, Chiba N, Chen S, Ramanujan V K, Wolf A J, Vergnes L, Ojcius D M, Rentsendorj A, Vargas M, Guerrero C, Wang Y, Fitzgerald K A, Underhill D M, Town T and Arditi M

  Immunity; 2012 Mar 23. 36 (3):401-14.

• Mitochondria Distinguish Granule-Stored from de novo Synthesized Tumor Necrosis Factor Secretion in Human Mast Cells

  Zhang B, Weng Z, Sismanopoulos N, Asadi S, Therianou A, Alysandratos K D, Angelidou A, Shirihai O and Theoharides T C

  Int Arch Allergy Immunol; 2012 Apr 27. 159 (1):23-32.

• Disruption of fnip1 reveals a metabolic checkpoint controlling B lymphocyte development

  Park H, Staehling K, Tsang M, Appleby M W, Brunkow M E, Margineantu D, Hockenbery D M, Habib T, Liggitt H D, Carlson G and Iritani B M

  Immunity; 2012 May 25. 36 (5):769-781.

• The Sedoheptulose Kinase CARKL Directs Macrophage Polarization through Control of Glucose Metabolism

  Haschemi A, Kosma P, Gille L, Evans C R, Burant C F, Starkl P, Knapp B, Haas R, Schmid J A, Jandl C, Amir S, Lubec G, Park J, Esterbauer H, Bilban M, Brizuela L, Pospisilik J A, Otterbein L E and Wagner O

  Cell Metab; 2012 Jun 6. 15 (6):813-826.

• Commitment to glycolysis sustains survival of nitric oxide-producing inflammatory dendritic cells

  Everts B, Amiel E, van der Windt G J, Freitas T C, Chott R, Yarasheski K E, Pearce E L and Pearce E J

  Blood; 2012 Aug 16. 120 (7):1422-31.

• Stomatin-like Protein 2 Deficiency in T Cells Is Associated with Altered Mitochondrial Respiration and Defective CD4+ T Cell Responses

  Christie D A, Mitsopoulos P, Blagih J, Dunn S D, St-Pierre J, Jones R G, Hatch G M and Madrenas J

  J Immunol; 2012 Nov 1. 189 (9):4349-4360.

• Mitochondrial dysfunction in osteoarthritis is associated with a down-regulation of superoxide dismutase 2

  Gavriilidis C, Miwa S, von Zglinicki T, Taylor R W and Young D A

  Arthritis Rheum; 2013 Feb 1. 65 (2):378-87.

• Mitochondria Are Required for Antigen-Specific T Cell Activation through Reactive Oxygen Species Signaling

  Sena L A, Li S, Jairaman A, Prakriya M, Ezponda T, Hildeman D A, Wang C R, Schumacker P T, Licht J D, Perlman H, Bryce P J and Chandel N S

  Immunity; 2013 Feb 21. 38 (2):225-236.

• Single-wall carbon nanohorns inhibited activation of microglia induced by lipopolysaccharide through blocking of Sirt3

  Li L, Zhang J, Yang Y, Wang Q, Gao L, Chang T, Zhang X, Xiang G, Cao Y, Shi Z, Zhao M and Gao G

  Nanoscale Res Lett; 2013 Feb 22. 8 (1):100.

• Methods for assessing mitochondrial function in diabetes

  Perry C G, Kane D A, Lanza I R and Neufer P D

  Diabetes; 2013 Apr 1. 62 (4):1041-1053.

• The MicroRNA miR-181 Is a Critical Cellular Metabolic Rheostat Essential for NKT Cell Ontogenesis and Lymphocyte Development and Homeostasis

  Henao-Mejia J, Williams A, Goff L A, Staron M, Licona-Limon P, Kaech S M, Nakayama M, Rinn J L and Flavell R A

  Immunity. 2013 Apr 24; [Epub ahead of print].

• Sterol regulatory element-binding proteins are essential for the metabolic programming of effector T cells and adaptive immunity

  Kidani Y, Elsaesser H, Hock M B, Vergnes L, Williams K J, Argus J P, Marbois B N, Komisopoulou E, Wilson E B, Osborne T F, Graeber T G, Reue K, Brooks D G and Bensinger S J

  Nature immunology; 2013 May 1. 14 (5):489-499.

Technical Posters

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• The Influence of Type II Diabetes on the Immune Response in Obese Adults

  Heather A. Paich, Patricia A. Sheridan, Terry L. Noah, Samuel S. Weir, and Melinda A. Beck

  Experimental Biology 2013
  April 20-24, 2013
  Boston, MA

• Specific Control of T Cell Effector Function by Warburg Metabolism

  CHIH-HAO CHANG

  Keystone Symposia, Metabolic Control of Inflammation and Immunity
  January 21-26, 2013
  Breckenridge, CO

• Dissecting the Role of Energy Metabolism in the Familial Autoinflammatory Disease TRAPS (TNF Receptor-Associated Periodic Syndrome)

  Martin Pelletier, Leah Billingham, Cornelia Cudrici, Daniel L. Kastner and Richard M. Siegel

  Keystone Symposia, Metabolic Control of Inflammation and Immunity
  January 21-26, 2013
  Breckenridge, CO

• Caspase-1 regulates mitochondrial metabolism and ROS production in mouse hepatocytes

  Qian Sun, Timothy Billiar, Melanie Scott

  Keystone Symposia, Metabolic Control of Inflammation and Immunity
  January 21-26, 2013
  Breckenridge, CO

• CD8 Memory T Cells Have A Bioenergetic Advantage That Underlies Their 'Rapid Recall' Ability

  Gerritje J.W. van der Windt, David O'Sullivan, Bart Everts, Stanley Ching-Cheng Huang, Michael D. Buck, Jonathan D. Curtis, Chih-Hao Chang, Amb

  Keystone Symposia, Metabolic Control of Inflammation and Immunity
  January 21-26, 2013
  Breckenridge, CO

• A new paradigm of CD4+ effector, memory and regulatory T cell differentiation

  Adam Waickman, Chirag Patel, Jonathan Powell

  Keystone Symposia, Metabolic Control of Inflammation and Immunity
  January 21-26, 2013
  Breckenridge, CO

• The role of nitric oxide as a regulator of cellular bioenergetics in lipopolysaccharide-stimulated macrophage

  Zhen Ding, Anne R. Diers and Neil Hogg

  Society for Free Radical Biology and Medicine (SFRBM 2012)
  November 15-18, 2012
  San Diego, CA

• Fatty Acid Oxidation Is Essential for Egg Production by the Parasitic Flatworm Schistosoma mansoni

  Stanley Ching-Cheng Huang, Tori C. Freitas, Eyal Amiel, Bart Everts, James B. Lok, Edward J. Pearce

  Molecular and Cellular Biology of Helminth Parasites VII 2012
  September 2-7, 2012
  Hydra, GR

• Metabolic regulation of CD8 T cell memory formation

  G.J.W. van der Windt, C.H. Chang, J.D. Curtis, and E.L. Pearce

  Keystone Symposia: Mitochondrial Dynamics and Function
  March 19-24, 2012
  Banff, Alberta, CA

• Mitochondrial dysfunction and oxidative damage in Osteoarthritis

  Christos Gavriilidis, Robert W Taylor, David A Young

  British Society for Matrix Biology Autumn Meeting, Matrix signals! Cell-matrix interactions in health and disease
  September 8-9, 2011
  Newcastle, UK

Webinars

Sort by: Title | Speaker | Date
• 

  Dissecting the role of energy metabolism and mitochondrial ROS in the familial autoinflammatory disease TRAPS

  Martin Pelletier, PhD, NIAMS/National Institutes of Health
  April 25, 2013

• 

  Mitochondrial Respiratory Capacity Is a Critical Regulator of CD8+ T Cell Memory Development

  Erika Pearce, PhD, Washington University School of Medicine
  St. Louis, MO
  February 14, 2012

• 

  XF Glycolysis Stress Test Assesses Glycolysis and Glycolytic Capacity in Cells

  Min Wu, PhD, Seahorse Bioscience
  August 30, 2011

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