Applications in Aging

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App Notes & Tech Briefs

Screening for Mitochondrial Biogenesis and Toxicity

A sensitive and robust cell-based screening assay.

Mitochondrial damage compromises ATP production and consequentially cellular viability. Given the central role that mitochondria play in regulating cellular function, drugs that undermine mitochondrial function frequently elicit toxic effects...
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Publications

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Ectopic lipid deposition and the metabolic profile of skeletal muscle in ovariectomized mice
Jackson K C, Wohlers L M, Lovering R M, Schuh R A, Maher A C, Bonen A, Koves T R, Ilkayeva O, Thomson D M, Muoio D M and Spangenburg E E
Am J Physiol Regul Integr Comp Physiol; 2013 Feb 1.
Review: Quantifying Mitochondrial Dysfunction in Complex Diseases of Aging
Horan M P, Pichaud N and Ballard J W
J Gerontol A Biol Sci Med Sci; 2012 Oct 1. 67 (10):1022-35.
GSK3 inactivation is involved in mitochondrial complex IV defect in transforming growth factor (TGF) beta1-induced senescence
Byun H O, Jung H J, Seo Y H, Lee Y K, Hwang S C, Seong Hwang E and Yoon G
Exp Cell Res; 2012 Sep 10. 318 (15):1808-19.
ATP13A2 mutations impair mitochondrial function in fibroblasts from patients with Kufor-Rakeb syndrome
Grunewald A, Arns B, Seibler P, Rakovic A, Munchau A, Ramirez A, Sue C M and Klein C
Neurobiol Aging; 2012 Aug 1. 33 (8):1843.e.1-7.
Rationale and Design of MAGNET (Mitochondria-AGing in NorthErn Taiwan) Study: A Community-based Cohort Investigating Mitochondria-related Aging and Cardiovascular Diseases in Suburban Areas of Northern Taiwan
Hung C-L, Wu Y-J, Liu C-C, Chi B-C, Chang S-S, Wang L-Y, Yeh H-I and Wei Y-H
International Journal of Gerontology; 2012 Jun 8. 6 (2):122-126.
Short-term calorie restriction enhances skeletal muscle stem cell function
Cerletti M, Jang Y C, Finley L W, Haigis M C and Wagers A J
Cell Stem Cell; 2012 May 4. 10 (5):515-519.
Oncogene-induced senescence results in marked metabolic and bioenergetic alterations
Quijano C, Cao L, Fergusson M M, Romero H, Liu J, Gutkind S, Rovira, II, Mohney R P, Karoly E D and Finkel T
Cell Cycle; 2012 Apr 1. 11 (7):1383-1392.
Ethanol withdrawal hastens the aging of cytochrome c oxidase
Jung M E, Ju X, Metzger D B and Simpkins J W
Neurobiol Aging; 2012 Mar 1. 33 (3):618.e21-32.
Resveratrol ameliorates aging-related metabolic phenotypes by inhibiting cAMP phosphodiesterases
Park S J, Ahmad F, Philp A, Baar K, Williams T, Luo H, Ke H, Rehmann H, Taussig R, Brown A L, Kim M K, Beaven M A, Burgin A B, Manganiello V and Chung J H
Cell; 2012 Feb 3. 148 (3):421-433.
The role of nuclear lamin B1 in cell proliferation and senescence
Shimi T, Butin-Israeli V, Adam S A, Hamanaka R B, Goldman A E, Lucas C A, Shumaker D K, Kosak S T, Chandel N S and Goldman R D
Genes Dev; 2011 Dec 15. 25 (24):2579-2593.
Identification and characterization of a functional mitochondrial angiotensin system
Abadir P M, Foster D B, Crow M, Cooke C A, Rucker J J, Jain A, Smith B J, Burks T N, Cohn R D, Fedarko N S, Carey R M, O'Rourke B and Walston J D
Proc Natl Acad Sci U S A; 2011 Sep 6. 108 (36):14849-14854.
Glutathionylation acts as a control switch for uncoupling proteins UCP2 and UCP3
Mailloux R J, Seifert E L, Bouillaud F, Aguer C, Collins S and Harper M E
J Biol Chem; 2011 Jun 17. 286 (24):21865-21875.
Intrinsic bioenergetic properties and stress sensitivity of dopaminergic synaptosomes
Choi S W, Gerencser A A, Lee D W, Rajagopalan S, Nicholls D G, Andersen J K and Brand M D
J Neurosci; 2011 Mar 23. 31 (12):4524-4534.
Loss of manganese superoxide dismutase leads to abnormal growth and signal transduction in mouse embryonic fibroblasts
Zhang Y, Zhang H M, Shi Y, Lustgarten M, Li Y, Qi W, Zhang B X and Van Remmen H
Free Radic Biol Med; 2010 Nov 1. 49 (8):1255-1262.
Regulation of skeletal muscle oxidative capacity and insulin signaling by the mitochondrial rhomboid protease PARL
Civitarese A E, MacLean P S, Carling S, Kerr-Bayles L, McMillan R P, Pierce A, Becker T C, Moro C, Finlayson J, Lefort N, Newgard C B, Mandarino L, Cefalu W, Walder K, Collier G R, Hulver M W, Smith S R and Ravussin E
Cell Metab; 2010 May 5. 11 (5):412-426.
Bmi1 regulates mitochondrial function and the DNA damage response pathway
Liu J, Cao L, Chen J, Song S, Lee I H, Quijano C, Liu H, Keyvanfar K, Chen H, Cao L Y, Ahn B H, Kumar N G, Rovira, II, Xu X L, van Lohuizen M, Motoyama N, Deng C X and Finkel T
Nature; 2009 May 21. 459 (7245):387-392.
Mitochondrial dysfunction and oxidative stress mediate the physiological impairment induced by the disruption of autophagy
Wu J J, Quijano C, Chen E, Liu H, Cao L, Fergusson M M, Rovira, II, Gutkind S, Daniels M P, Komatsu M and Finkel T
Aging (Albany NY); 2009 Apr 1. 1 (4):425-437.

Technical Posters

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Cockayne syndrome group B protein prevents the accumulation of damaged mitochondria by promoting mitochondrial autophagy
Morten Scheibye-Knudsen et al., Vilhelm A. Bohr
Aging and Diseases of Aging (S2), Keystone symposium
October 22-27, 2012
Tokyo, Japan
Long term culture of lung fibroblasts is accompanied by increased doubling time, mitochondrial hyperfusion, and altered oxygen consumption prior to the...
Daniel E. Hurley, Manish S. Bharadwaj, Jordan Strober, Anthony J. A. Molina
Mitochondria and Metabolism
June 12-14, 2012
Philadelphia, PA
Mitochondrial ribosomal proteins regulate longevity in three species through the mitochondrial unfolded protein response
R.H. Houtkooper, L.Mouchiroud, M.Beekman, E.Katsyuba, J.Deelen, D.Ryu, G.Knott, A.G.Uitterlinden, A.Hofman, P.E.Slagboom, R.W.Williams, J. Auwerx
Keystone Symposia: Mitochondrial Dynamics and Function
March 19-24, 2012
Banff, Alberta, CA
The Homeobox Protein Prox1 is a Negative Modulator of ERR[Alpha]/PGC-1[Alpha] Bioenergetic Functions
Alexis Charest-Marcotte, Catherine R. Dufour, Brian J. Wilson, Annie M. Tremblay, Lillian J. Eichner, Daniel H. Arlow, Vamsi K. Mootha and Vincent Gigu
Nuclear Receptors: Development, Physiology and Disease
March 21-26, 2010
Keystone, CO, US
Testing modulators of uncoupling protein (UCP) function in intact HEK293 cells using plate-based respirometry and membrane potential sensitive fluorescent probes
Martin Jastroch, Akos A. Gerencser, and Martin D. Brand
Molecular & Cellular Bioenergetics Gordon Research Conference
June 10, 2009
Keystone, CO

Webinars

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Biochemical analysis in C. elegans as a model for metabolic disease and aging
Laurent Mouchiroud, PhD , École Polytechnique Fédérale de Lausanne
Riekelt H. Houtkooper, PhD, École Polytechnique Fédérale de Lausanne
November 30, 2011
Spare Respiratory Capacity and the Bioenergetics of the Nerve Terminal
David Nicholls, PhD, FRSE, Buck Institute for Age Research & Lund University
December 3, 2009
The Bioenergetics of Obesity: New Approaches to Characterize Genes Affecting Energy Balance in Mouse Models
Karen Reue, PhD, David Geffen School of Medicine at UCLA
April 30, 2009
The Response of the Mitochondrion to Oxidative Stress in Cardiovascular Cells
Victor Darley-Usmar, PhD, University of Alabama at Birmingham
November 11, 2008
Thyroid Hormone Deiodination is a Key Switch for Energy Expenditure
Antonio C. Bianco, MD, PhD, Brigham and Women’s Hospital & Harvard Medical School
March 25, 2008
Bioenergetic Consequences of Mitochondrial Fusion and Fission
Orian Shirihai, MD, PhD , Tufts University School of Medicine
January 30, 2008

Mitochondrial dysfunction is of central concern in the ageing process. Increasing age correlates with an accumulation of oxidative damage and somatic mitochondrial DNA (mtDNA) mutations and a decline in oxidative phosphorylation.

The XF Analyzer generates data that can provide new understanding in relation to aging and mitochondrial dysfunction, by allowing the measurement of cellular metabolism in real-time, in a microplate. By measuring the oxygen consumption rate (OCR) - a measure of mitochondrial respiration; as well as the extracellular acidification rate (ECAR) - a measure of glycolysis, you will be provided with the most physiologically relevant in vitro measurement for your bioenergetic studies.